Unlike other frogs in the genus that are predominantly stream dwelling, the new miniature frogs were found under damp forest leaf litter or marsh vegetation. Scientists were surprised by the relative abundance of these previously unknown species at their collection localities. "In fact, the miniature species are locally abundant and fairly common but they have probably been overlooked because of their extremely small size, secretive habitats and insect-like calls," says Sonali Garg who undertook this study as part of her PhD research at University of Delhi.
In the lab, the newly sampled frogs were confirmed as new species by using an integrated taxonomic approach that included DNA studies, detailed morphological comparisons and bioacoustics. Evidence from these multiple sources confirmed that the diversity of Night frogs is higher than previously known and particularly remarkably for the miniaturized forms. Previously, the Night Frog genus composed of 28 recognized species of which only three were miniature-sized.
Now the total number of known Nyctibatrachus species has increased to 35, of which 20 percent are diminutive in size. This frog genus is endemic to the Western Ghats of India and represents an ancient group of frogs that diversified on the Indian landmass approximately 70-80 million years ago.
The discovery of several new species of ancient origin can provide useful insights into the evolution of endemic frog lineages in the Western Ghats, which is a leading amphibian hotspot. The past decade has witnessed an exponential increase in the number of new amphibian species described from this region. Of the total new species of amphibians (1581) described globally between the years 2006-2015, the highest number were from the Brazilian Atlantic Forest (approximately 182) followed by the Western Ghats-Sri Lanka biodiversity hotspot (approximately 159), with 103 species described alone from the Western Ghats region.
However, the future of many of the new species may be bleak. All the newly described species are currently known only from single localities in the southern Western Ghats, and some lie outside Protected areas. Researchers found the Radcliffe's Night frog and the Kadalar Night Frog inside private or state-owned plantation areas facing threats such as habitat disturbance, modification and fragmentation. The Athirappilly Night Frog was found in close vicinity to the Athirappilly waterfalls and the Sabarimala Night Frog near the Sabarimala pilgrimage centre, both of which are disturbed by anthropogenic activities. "Over 32 percent, that is one-third of the Western Ghats frogs are already threatened with extinction. Out of the seven new species, five are facing considerable anthropogenic threats and require immediate conservation prioritization," says Prof SD Biju, who led the new study and has also formally described over 80 new species of amphibians from India
Sonali Garg, Robin Suyesh, Sandeep Sukesan, SD Biju. Seven new species of Night Frogs (Anura, Nyctibatrachidae) from the Western Ghats Biodiversity Hotspot of India, with remarkably high diversity of diminutive forms. PeerJ, 2017; 5: e3007 DOI: 10.7717/peerj.300718>